Each operation, according to Medalla, consists of many sequential steps that are crucial and important if success is to be achieved. Despite the electricity fluctuation in the university when MARID visited the university for two days, she shared how mushroom technology functions in their laboratory and underground passageways:
A. Propagation of pure culture
During the preparation of the culture media, peel and weigh the 200 grams of fresh, hale and hearty potatoes. Dice these potatoes to about two-centimeter cubes. Boil one-liter distilled water and add these diced potatoes. Let them simmer for ten minutes or until they are soft enough to be eaten. Strain off these diced potato through cheesecloth, and restore the volume of the potato decoction (broth) to one liter by simply adding distilled water. Bring the potato decoction to boil, and add 20 grams of agar. Stir the mixture until the agar dissolves. Add 20 grams of dextrose powder and stir until it also dissolves.
Pour and distribute 40 to 50 milliliters of the mixture into empty bottles. Plug the mouth of the bottles with cotton, cover with paper, and tie with rubber band. Sterilize them in pressure cooker at 15 pounds for 15 to 20 minutes, and allow the pressure to drop down to zero pounds per square inch. Take out the sterilized culture media, and slant the culture media bottles.
Then isolate the pure culture using the tissue culture. This method is done through selecting a young and healthy mushroom that is disinfected with 70 percent alcohol by rubbing a cotton swab. With a sterilized scalpel, cut approximately one-centimeter cube of the tissue and plant on the slanted agar with the use of sterilized forceps or scalpel. Incubate at room temperature for ten to 14 days or until the media is fully impregnated with the mycelia.
B. Production of the spawn
Wash thoroughly the grains in tap water. Transfer in clean casserole, and add water till one inch above the level of grains. Boil until the grains are about to burst. Cool the grains by spreading in a nylon cloth or line screen and allow the water to drain off leaving the grains just damp (65 to 70 percent moisture). Distribute grains equally in bottles, plug with cotton and cover them with paper or foil, and support them with rubber band. Sterilize at 15 pounds for an hour. Cool the bottles at room temperature; aseptically inoculate with young and vigorous culture of the mushroom mold. Incubate at room temperature until the grains are fully impregnated with the mushroom molds.
C. Production of the fruiting bags (growing method)
At the preparation of the composted sawdust medium, mix thoroughly the composted sawdust medium composed of 78 percent sawdust, 20 percent class A rice bran, one percent calcium carbonate or lime and a percent of washed sugar. Add tap water until the mixture attains 65 to 70 percent moisture, which is determined by pressing a handful of mixture in the hand and no water should run off in between fingers and the materials should stay in form after releasing the pressure.
Pile the substrate or mixture in a pyramidal form. Cover with plastic sheet for a period of five days with turning every after two days, repiling and returning the plastic cover again. On the fifth day or after the second turning, aerate the piled materials by spreading thinly in a shaded area to remove the toxic gases that may have been produced during the period of composting. After the acidic smell has gone, check and adjust the moisture content making sure that it is 65 to 70 percent.
Pack the substrate in not-so-loose-or-not-so-compact bag. Collect the upper part of the bag and pass it through a plastic ring, and pull the plastic ring down thus making the mouth of the bag. Plug each bag with cotton, cover it with paper, and tie with rubber band. Sterilize 15 pounds for an hour and a half, or the bags may be subjected to steaming process. The period of steaming depends on the load of the container.
To propagate the mushrooms, transfer the sterilized and cooled bags inside the inoculation room, which has been previously disinfected. Aseptically inoculate each bag with the mother spawn. Transfer the spawned bags in the incubation house, and incubate at 25 to 28 degrees Celcius for a month or until fully impregnated with the mushroom mold.
Once the bags are fully ramified, allow them to undergo maturation period by extending incubation by another month. The matured bags are then ready to open. Open the bags carefully by cutting the plastic below the neck, and water them abundantly but carefully. The floor and the walls of the house should also be watered.
On the third day, pinheads or primordial will develop. In this case, do not water the bags but the walls and the floor. Harvest period is expected on the fourth and the fifth day and harvesting goes for two to three days. After fruiting, the bags should rest for five to seven days. During rest period, monitor the temperature (25 to 28 degrees Celcius), relative humidity (85 to 90 percent) and moisture (65 to 70 percent). After each rest period, normal flushing or fruiting will follow and the cycle is repeated. The complete fruiting cycle lasts for two to three months.
Author: Jesse John Edep, Marid Digest